AGA eDigest

July 27, 2006

 

Mutant Gene Causes Congenital Malabsorptive Diarrhea

 

A newly discovered disorder characterized by malabsorptive diarrhea and a lack of intestinal enteroendocrine cells is caused by mutations in Neurogenin-3 (NEUROG3), reports last week's New England Journal of Medicine.

 

NEUROG3 is expressed in endocrine progenitor cells and is required for endocrine-cell development in the pancreas and intestine. The NEUROG3 gene is therefore a candidate for the cause of a newly discovered autosomal recessive disorder which is characterized by generalized malabsorption and a paucity of enteroendocrine cells. Researchers from California screened genomic DNA from three unrelated patients with sparse enteroendocrine cells for mutations of NEUROG3. The team then tested the ability of the observed mutations to alter NEUROG3 function, using in vitro and in vivo assays.

 

The investigators found that patients had few intestinal enteroendocrine cells positive for chromogranin A. However, the patients had normal numbers of Paneth's, goblet, and absorptive cells. The team identified two homozygous mutations in NEUROG3. Both of these mutations rendered the NEUROG3 protein unable to activate NEUROD1, a downstream target of NEUROG3. The investigators noted that the mutations compromised the ability of NEUROG3 to bind to an E-box element in the NEUROD1 promoter. – Newsfeed from GastroHep.com

 

New England Journal of Medicine; 2006: 355(3): 270-80